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human ankrd49 coding sequence cds  (TaKaRa)


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    TaKaRa human ankrd49 coding sequence cds
    Human Ankrd49 Coding Sequence Cds, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 546 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pdsred2+n1/pDsRed2-N1+Vector/pm41821002-124-10-21
    Average 95 stars, based on 546 article reviews
    human ankrd49 coding sequence cds - by Bioz Stars, 2026-10
    95/100 stars

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    Plasmid Preparation:

    Article Title: Functional characterization of BRCA1 variants of unknown significance using homologous recombination repair assays.
    Article Snippet: .. Plasmid pcDNA3 was purchased in Invitrogen (InvitrogenTM V79020) and pDSRed2-N1, a plasmid encoding the red fluorescent protein DsRed2, was purchased in Clontech. .. Site-specific point mutations were generated using the QuickChange II XL Site-Directed Mutagenesis Kit from Agilent Technologies.

    Article Title: Functional characterization of BRCA1 variants of unknown significance using homologous recombination repair assays
    Article Snippet: .. Plasmid pcDNA3 was purchased in Invitrogen (InvitrogenTM V79020) and pDSRed2-N1, a plasmid encoding the red fluorescent protein DsRed2, was purchased in Clontech. .. Site-specific point mutations were generated using the QuickChange II XL Site-Directed Mutagenesis Kit from Agilent Technologies.

    Article Title: Osteocalcin-expressing neutrophils from skull bone marrow exert immunosuppressive and neuroprotective effects after TBI.
    Article Snippet: .. Förster resonance energy transfer (FRET) analysis of intercellular interaction between A2AR and mGluR5 Then the fragments of A2AR (NCBI ID: 11540) were subcloned into the pDsRed2-N1 (Cat no. 632406, Clontech) vectors after digestion with Xho I and BamH I and the resulting plasmid was named as A2AR-RFP. .. The mGluR5 (NCBI ID: 108071) were achieved like above and was subcloned into pEGFP-N1 (VT1110, YouBio Technology) vectors after digestion with EcoR I and BamH I and the resulting plasmid was named asmGluR5-EGFP.

    Fluorescence:

    Article Title: miR‑27a‑3p upregulation by p65 facilitates cervical tumorigenesis by increasing TAB3 expression and is involved in the positive feedback loop of NF‑κB signaling.
    Article Snippet: .. For 3'UTR EGFP fluorescence reporter assays, the HeLa cells were seeded in 24‐well plates and transfected with the wild‐type or mutated form of the reporter vectors (500 ng/well) with/without the overexpression of miR‐27a‐3p. pDsRed2‐N1 (100 ng/well, Clontech; Takara Bio USA) co‐transfected into each well for normalization. .. At 48 h following transfection, the cells were lysed with RIPA buffer and the fluorescence intensi‐ ties of EGFP and RFP were determined using a microplate reader (Tecan Spark, Tecan Group, Ltd.).

    Transfection:

    Article Title: miR‑27a‑3p upregulation by p65 facilitates cervical tumorigenesis by increasing TAB3 expression and is involved in the positive feedback loop of NF‑κB signaling.
    Article Snippet: .. For 3'UTR EGFP fluorescence reporter assays, the HeLa cells were seeded in 24‐well plates and transfected with the wild‐type or mutated form of the reporter vectors (500 ng/well) with/without the overexpression of miR‐27a‐3p. pDsRed2‐N1 (100 ng/well, Clontech; Takara Bio USA) co‐transfected into each well for normalization. .. At 48 h following transfection, the cells were lysed with RIPA buffer and the fluorescence intensi‐ ties of EGFP and RFP were determined using a microplate reader (Tecan Spark, Tecan Group, Ltd.).

    Over Expression:

    Article Title: miR‑27a‑3p upregulation by p65 facilitates cervical tumorigenesis by increasing TAB3 expression and is involved in the positive feedback loop of NF‑κB signaling.
    Article Snippet: .. For 3'UTR EGFP fluorescence reporter assays, the HeLa cells were seeded in 24‐well plates and transfected with the wild‐type or mutated form of the reporter vectors (500 ng/well) with/without the overexpression of miR‐27a‐3p. pDsRed2‐N1 (100 ng/well, Clontech; Takara Bio USA) co‐transfected into each well for normalization. .. At 48 h following transfection, the cells were lysed with RIPA buffer and the fluorescence intensi‐ ties of EGFP and RFP were determined using a microplate reader (Tecan Spark, Tecan Group, Ltd.).

    Amplification:

    Article Title: Recombinant constructs and transgenic fluorescent ornamental fish therefrom
    Article Snippet: .. Red fluorescent protein gene, DsRed2; green fluorescent protein gene, ZsGreen1 and yellow fluorescent protein gene, ZsYellow1 were amplified by PCR from pDsRed2-N1, pZsGreen1-N1 and pZsYellow1-N1 (Clontech Inc., Matz. et al., 1999) respectively and cloned into pBluescript II SK (−) zMLC2 and pUC18-carp β-actin such that the promoter was operably linked to the fluorescent gene. ..

    Polymerase Chain Reaction:

    Article Title: Recombinant constructs and transgenic fluorescent ornamental fish therefrom
    Article Snippet: .. Red fluorescent protein gene, DsRed2; green fluorescent protein gene, ZsGreen1 and yellow fluorescent protein gene, ZsYellow1 were amplified by PCR from pDsRed2-N1, pZsGreen1-N1 and pZsYellow1-N1 (Clontech Inc., Matz. et al., 1999) respectively and cloned into pBluescript II SK (−) zMLC2 and pUC18-carp β-actin such that the promoter was operably linked to the fluorescent gene. ..

    Article Title: OpsinLW2 serves as a circadian photoreceptor in the entrainment of circadian locomotor rhythm of a firebrat.
    Article Snippet: Photic entrainment is an essential function of the circadian clock, which enables organisms to set the appropriate timing of daily behavioral and physiological events.. Recent studies have shown that the mechanisms of the circadian clock and photic entrainment vary among insect species.. This study aimed to elucidate the circadian photoreceptors necessary for photic entrainment in firebrats Thermobia domestica, one of the most primitive apterygote insects.

    Clone Assay:

    Article Title: Recombinant constructs and transgenic fluorescent ornamental fish therefrom
    Article Snippet: .. Red fluorescent protein gene, DsRed2; green fluorescent protein gene, ZsGreen1 and yellow fluorescent protein gene, ZsYellow1 were amplified by PCR from pDsRed2-N1, pZsGreen1-N1 and pZsYellow1-N1 (Clontech Inc., Matz. et al., 1999) respectively and cloned into pBluescript II SK (−) zMLC2 and pUC18-carp β-actin such that the promoter was operably linked to the fluorescent gene. ..

    other:

    Article Title: BET bromodomain inhibition potentiates radiosensitivity in models of H3K27-altered diffuse midline glioma
    Article Snippet: Transfections were performed using Lipofectamine 2000 (ThermoFisher Scientific 11668027).

    Förster Resonance Energy Transfer:

    Article Title: Osteocalcin-expressing neutrophils from skull bone marrow exert immunosuppressive and neuroprotective effects after TBI.
    Article Snippet: .. Förster resonance energy transfer (FRET) analysis of intercellular interaction between A2AR and mGluR5 Then the fragments of A2AR (NCBI ID: 11540) were subcloned into the pDsRed2-N1 (Cat no. 632406, Clontech) vectors after digestion with Xho I and BamH I and the resulting plasmid was named as A2AR-RFP. .. The mGluR5 (NCBI ID: 108071) were achieved like above and was subcloned into pEGFP-N1 (VT1110, YouBio Technology) vectors after digestion with EcoR I and BamH I and the resulting plasmid was named asmGluR5-EGFP.



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